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Programme features


During an initial general introduction, the schedule will be explained and the students will be assigned to different teams. Given the background diversity of the attendees, several introductory lectures will aid the participants to focus on their technical aspects during training. The course will focus on the following techniques: NMR, MALDI-TOF, spin labelling, calorimetry, dynamic light scattering, SPR and data processing.

  • NMR (Nuclear Magnetic Resonance)

    Protein-observer experiments will rely on 2D 1H-15N correlation to determine the location of the binding site and estimation of the Kd according to Chemical Shift Perturbation, CSP. Additionally, Paramagnetic Relaxation Enhancements, PRE, will be acquired and analysed.

    On the other hand, several ligand-observed experiments as tr-NOESY, STD and Water LOGSY will be performed and compared to achieve real understanding of the different methods. Processing and analysis of the acquired experiments will be also performed in parallel sessions.
  • MALDI-TOF (Soft ionization mass spectroscopy)

    Analysis of biomolecular standards and measurement of the stoichiometry of non-covalent protein-protein interaction will be performed.

  • Spin labelling

    Standard spin labelled samples will be prepared to measure Paramagnetic Relaxation Enhancements, PRE, by NMR

  • ITC (Isothermal titration Calorimetry)

    Calorimetric measurements of binding constants of protein-protein and protein-ligand interactions will be performed and analyses as well as protein stability analyses upon ligand binding by Differential Scanning Calorimetry, DSC.

  • DLS (Dynamic Light scattering)
    Sample test by Dynamic Light scattering, DLS, will be performed and analysed.

  • SPRi (Surface Plasmon Resonance)
    Surface Plasmon Resonance,SPRi, will be performed using standard protein-DNA samples and sensorgram analysis will be analysed.

  • Complementary techniques as EPR, electron microscopy and SAXS will be also introduced as lectures during the course.



600 MHz NMR Spectrometer


Surface Plasmon Resonance imaging


Calorimetry


MALDI-TOF mass spectrometer


Light Scattering

Circular Dischroism
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